Optical modeling of an ultrathin scanning fiber endoscope, a preliminary study of confocal versus non-confocal detection.
نویسندگان
چکیده
An optical model of an ultrathin scanning fiber endoscope was constructed using a non-sequential ray tracing program and used to study the relationship between fiber deflection and collection efficiency from tissue. The problem of low collection efficiency of confocal detection through the scanned single-mode optical fiber was compared to non-confocal cladding detection. Collection efficiency is 40x greater in the non-confocal versus the confocal geometry due to the majority of rays incident on the core being outside the numerical aperture. Across scan angles of 0 to 30o, collection efficiency decreases from 14.4% to 6.3% for the non-confocal design compared to 0.34% to 0.10% for the confocal design. Non-confocality provides higher and more uniform collection efficiencies at larger scan angles while sacrificing the confocal spatial filter.
منابع مشابه
Compact optical design for dual-axes confocal endoscopic microscopes
Here we describe a simple optical design for a MEMS-based dual-axes fiber optic confocal scanning microscope that has been miniaturized for handheld imaging of tissues, and which is capable of being further scaled to smaller dimensions for endoscope compatibility while preserving it’s field-of-view (FOV), working distance, and resolution. Based on the principle of parallel beams that are focuse...
متن کاملVariations of the Normal Human Limbal Stem Cells Detected by In Vivo Confocal Microscopy
Background To report normal variations of the limbal structures using in vivo laser scanning confocal microscopy. Methods: This was a retrospective study of fourteen eyes from 11 healthy individuals. Confocal imaging of cornea and limbus was performed using Heidelberg Retina Tomograph III Rostock Corneal Module. Results: The typical structure of the palisades of Vogt (POV) was detected ...
متن کاملConfocal Laser Scanning Microscopy for Detection of Schistosoma mansoni Eggs in the Gut of Mice
BACKGROUND The gold standard for diagnosing Schistosoma mansoni infections is the detection of eggs from stool or biopsy specimens. The viability of collected eggs can be tested by the miracidium hatching procedure. Direct detection methods are often limited in patients with light or early infections, whereas serological tests and PCR methods fail to differentiate between an inactive and persis...
متن کاملREVIEW Confocal scanning optical microscopy and its applications for biological specimens
Confocal scanning optical microscopy (CSOM) is a new optical microscopic technique, which offers significant advantages over conventional microscopy. In laser scanning optical microscopy (SOM), the specimen is scanned by a diffractionlimited spot of laser light, and light transmitted or reflected by the in-focus illuminated volume element (voxel) of the specimen, or the fluorescence emission ex...
متن کاملConfocal soft X-ray scanning transmission microscopy: setup, alignment procedure and limitations
Zone-plate-based scanning transmission soft X-ray microspectroscopy (STXM) is a well established technique for high-contrast imaging of sufficiently transparent specimens (e.g. ultrathin biological tissues, polymer materials, archaeometric specimens or magnetic thin films) with spatial resolutions in the regime of 20 nm and high spectroscopic or chemical sensitivity. However, due to the relativ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Optics express
دوره 13 19 شماره
صفحات -
تاریخ انتشار 2005